Rai P, Padala C, Poon V, et al. Statistical pattern matching facilitates the design of polyvalent inhibitors of anthrax and cholera toxins. Nature Biotech 2006 Apr 23 (early online publication) [Full text] Apr 24 NIH news releasehttp://www.niaid.nih.gov/news/newsreleases/2006/Pages/AMD_06.aspx In test-tube experiments, the antitoxin was 10,000 times more potent than single peptides, the NIH said. The NIH-supported research was done by a team led by Ravi S. Kane, PhD, of Rensselaer Polytechnic Institute in Troy, N.Y., and Jeremy Mogridge, PhD, of the University of Toronto. Their report was published Apr 23 in Nature Biotechnology. See also: The PA molecule has a number of sites that can bind to host cells, the NIH said. Therefore, Kane and colleagues created a molecule that can cling to PA at multiple sites. The protein-studded bubble they produced is called a peptide-functionalized liposome. “Just as a glove matches the shape of a hand more closely than a mitten, and so fits more snugly, the polyvalent inhibitor binds the toxin at multiple sites and is orders of magnitude more potent than an inhibitor that binds at a single site,” the agency said. “The multiple peptides on the functionalized liposome are arranged with the same average spacing as the binding sites of the PA molecule, which permits a firmer bond between the two, explains Dr Kane.” The researchers created a fatty bubble, studded with proteins, that binds to a key component of anthrax toxin at multiple sites, preventing it from attaching to host cells, the National Institutes of Health (NIH) said in a news release. “This novel approach to the design of anthrax antitoxin is an important advance, not only for the value it may have in anthrax treatment, but also because this technique could be used to design better therapies for cholera and other diseases,” said NIH Director Elias A. Zerhouni, MD. The investigators used the same liposome-based technique to develop a polyvalent inhibitor of cholera toxin, which also worked well in test-tube experiments, the NIH said. As explained in the NIH release, the toxin produced by Bacillus anthracis has three parts: a protein called protective antigen (PA), which binds to receptors on target cell surfaces, and two enzymes. When PA binds to a host cell, it creates a pore that enables the enzymes to enter the cell. Apr 25, 2006 (CIDRAP News) Scientists have come up with a new approach to neutralizing the toxin produced by anthrax bacteria and have tested it successfully in a small number of animals, according to a report published this week. The antitoxin was also tested in rats. When nine rats were simultaneously injected with anthrax toxin and relatively small doses of the new antitoxin, five of them remained healthy. When slightly higher doses of the inhibitor were used, eight of nine rats were protected. The journal article says the experiment was the first to show that a liposome-based polyvalent inhibitor works in animals. The team next plans to infect animals with B anthracis and allow the disease process to begin before treating them with the anthrax antitoxin, the agency said.
PORT OF SPAIN, Trinidad (CMC) – Reigning Caribbean Premier League champions Jamaica Tallawahs won their second on the trot when they beat Trinbago Knight Riders by four wickets here Wednesday night.Chasing 148 at Queen’s Park Oval, Tallawahs got home with four balls remaining to notch their second win in four days, following their opening loss to Barbados Tridents in Florida last weekend.Tallawahs were guided by Sri Lankan star and captain Kumar Sangakkara who top-scored with 47 while opening partner Lendl Simmons struck 38.Champion off-spinner Sunil Narine (2-21) and seamer Kevon Cooper (2-25) grabbed two wickets apiece.New Zealander Colin Munro had earlier top-scored with 41 as TKR, sent in, were dismissed for 147 with a ball to spare.Left-hander Darren Bravo punched 33 while opener Narine blasted 23, but seamers Odean Smith (3-20) and Kesrick Williams (2-36) snatched three wickets apiece to retard TKR’s progress.In fact, the hosts were cruising at 109 for two in the 10th over before suffering a dramatic collapse, losing their last eight wickets for a mere 38 runs.Narine had given the innings an energetic start, smacking a pair of fours and sixes in an 11-ball cameo, adding 33 for the first wicket with New Zealander Brendon McCullum (16).When Narine perished in the fifth over caught at mid-wicket by Simmons off Man-of-the-Match Williams, Munro and Bravo put TKR on top with a 57-run third-wicket stand.Munro struck five fours and two sixes off 25 balls while Bravo also faced 25 deliveries and counted two fours and two sixes.Once Munro holed out in the deep at the end of the 10th, however, the innings slumped into terminal decline.Tallawahs’ run chase was given an immediate boost when Sangakkara and Simmons put on 61 off 33 balls for the first wicket.While the left-handed Sangakkara was measured in his 41-ball knock which included seven fours, Simmons played with abandon, carving out four fours and three sixes off just 18 balls.Simmons was one of three wickets to fall for 32 runs in the space of 30 balls when he was bowled by Pakistan leg-spinner Shadab Khan in the sixth over but Tallawahs kept their focus to get home in the final over.
Four different area high school bowling teams made their way back into competition after two weeks of rest and practice during the holiday school break.Fayetteville-Manlius got the first action last week, and the girls Hornets handled West Genesee 3-0 in last Monday’s match at Green Lakes Bowl.It was particularly special for Shelby Miner. Starting with a 192, Miner tore it up with games of 250 and 240 to earn a season-best 682 series.Help came from Samantha Estock, whose 213 game completed a 527 series, and Kendra Elliot, who posted a 513 set as Hannah Smith earned a 467 series.F-M’s boys lost, 2-1, to West Genesee. Kevin Winschel shot a 627 series (high game 248) and Aidan Koss got a 616 set. The Wildcats got twin 604 series from Richard Wituszynski and Bobby Bidwell.Three days later, the Hornets went up against Baldwinsville at B’ville Sports Bowl. Five F-M boys bowlers shot series of 529 or better, but no one topped the 587 set from Koss as the Bees, who won 3-0, got a 695 series from Tanner Rozcyzko, plus solid series from Dylan Love (611) and Dylan Williams (605).On the girls side, Elliot shot 225, 191 and 193 in her 609 series, with Miner (470 series) beating out Smith (461 series). But the Bees still beat F-M 3-0, with Madison Hass shooting a 257 as part of a 646 series.Then F-M challenged Liverpool on Friday, but was unable to earn a point in either match against the Warriors despite some fine individual efforts.The boys Hornets had Winschel shoot a 627 series and Koss a 594 series against Liverpool, who saw Dylan Roberson shoot a 276 game in his 662 series as Josh Winzens had a 620 set.In the girls 3-0 loss, Miner shot a 545 series ahead of Estock (499 series) and Elliot (495 series), topped by the 619 set from Liverpool’s Ashley Hardy and 577 series from Makenzi Ormsby.East Syracuse Minoa ran up against Fulton last Wednesday, with the boys Spartans earning a big 2-1 victory over the Red Raiders.Tyler Talarico shot 10 strikes in his opening 288 game on his way to a 676 series. Brett Smith earned a 601 series, with Chris Bayly adding a 560 set as ESM overcame a 717 series from Fulton’s Mitch Donaldson.On the girls side, ESM fell to Fulton 3-0, with America Carhart’s 501 series by far the best among the Spartans. Alexis Ingersoll once more led the undefeated (8-0) Red Raiders, her 227 game leading to a 578 set.Up against Central Square on Thursday, ESM took a 3-0 loss, with Talarico getting a 637 series at Green Acres Bowl and Bayly adding a 567 series.Central Square’s Dimitri Queior earned a 709 series with games of 247, 226 and 236, helped by Jayden Allen (643 series) and Kevin Radzieski, whose 268 game kicked off a 604 series.Once again, the girls Spartans were unable to get a point during its 3-0 defeat to Central Square, though Carhart, helped by a 235 second game, improved to a 596 series, ahead of the 548 set by the Redhawks’ Victoria Ackley.There was a head-to-head clash last Tuesday at Strike-N-Spare Lanes between Christian Brothers Academy and Bishop Grimes, with the two sides splitting their matches.On the boys end, it was the Cobras blanking the Brothers 7-0, Grimes led by Joe Belton’s 499 series as Ben Schultz earned a 471 series. CBA got a 450 series from Garrett Bowman.CBA’s girls beat Grimes 7-0 to improve to 6-1 on the season as Eliana Occhino led the way, her 225 game in the middle of a 578 series. Cadence Malicek earned a three-game total of 502, just ahead of Rachel Farella’s 487 series.Staying in good form on Thursday, CBA’s girls handled Solvay 7-0, with Malicek’s 497 series capped by a 206 game. Sophie Schultz had a 449 series and Farella added a 419 set.But the CBA boys lost to Solvay by that same score, Tavish Johnston earning a 444 series for the Brothers, but far behind the Bearcats’ Russ Tarbell (575 series) and Ethan Bigelow (574 series) as Solvay improved to 6-0.Grimes’ boys swept Altmar-Parish-Williamstown 7-0 on Thursday, led this time by Schultz, whose 207 came in the middle of a 546 series. On Friday, both Grimes teams lost 7-0 to Homer and CBA fell 4-3 to APW.Share this:FacebookTwitterLinkedInRedditComment on this Story Tags: Bishop GrimesbowlingCBAESMF-M